Difference between revisions of "Part:BBa K208045"
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K208045 SequenceAndFeatures</partinfo> | <partinfo>BBa_K208045 SequenceAndFeatures</partinfo> | ||
| − | [[Image:GFP characterization.jpg]] [[Image:GFP_gel.jpg]] | + | [[Image:GFP characterization-1.jpg]] |
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| + | GFP activity is not observed in cells without the BBa K208045 BioBrick. GFP activity is also not seen in BBa K208045 containing cells with the lac repressor and no IPTG. Cells express the GFP when in cells that lack the lac repressor. These results demonstrate the functionality of this BBa K208045 BioBrick. | ||
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| + | [[Image:GFP_gel.jpg]] | ||
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| + | The bright band at 27 kD in the GFP+ sample corresponds to the GFP protein. The absence of this band in the GFP- sample further reinforces the functionality of the GFP construct. | ||
Revision as of 02:37, 22 October 2009
Lac Promoter/RBS/GFP/Term
GFP expression composite part using K208000 GFP.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 230
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 749
Illegal XhoI site found at 650 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
GFP activity is not observed in cells without the BBa K208045 BioBrick. GFP activity is also not seen in BBa K208045 containing cells with the lac repressor and no IPTG. Cells express the GFP when in cells that lack the lac repressor. These results demonstrate the functionality of this BBa K208045 BioBrick.
The bright band at 27 kD in the GFP+ sample corresponds to the GFP protein. The absence of this band in the GFP- sample further reinforces the functionality of the GFP construct.