Difference between revisions of "Part:BBa K5301029"

Latest revision as of 09:33, 1 October 2024

spNW15 expression pathway (T7)

This pathway expresses the SpyCatcher-NW15-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW15 to construct nanodiscs.

Usage and Biology

We used this pathway to express spNW15 by using T7 expression system in E.coli, and the expression of spNW15 can be induced by IPTG. For more information on spNW15, go to BBa_K5301013.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 571
Illegal SpeI site found at 523
Illegal PstI site found at 739
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 571
Illegal NheI site found at 139
Illegal NheI site found at 1175
Illegal SpeI site found at 523
Illegal PstI site found at 739
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 571
Illegal BglII site found at 627
Illegal BglII site found at 999
Illegal BamHI site found at 172
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 571
Illegal SpeI site found at 523
Illegal PstI site found at 739
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 571
Illegal SpeI site found at 523
Illegal PstI site found at 739
Illegal AgeI site found at 1102
1000
COMPATIBLE WITH RFC[1000]

@@ Line 9: / Line 9: @@
 We used this pathway to express spNW15 by using T7 expression system in <i>E.coli</i>, and the expression of spNW15 can be induced by IPTG. For more information on spNW15, go to <partinfo>BBa_K5301013</partinfo>.
+===Sequence and Features===
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'></span>
 <partinfo>BBa_K5301029 SequenceAndFeatures</partinfo>