Difference between revisions of "Part:BBa K5301025"

Latest revision as of 09:26, 1 October 2024

novel SEND system

A novel SEND system based on BBa_K4140013. VLP formed by PEG10 can carry GFP mRNA, and vesicles containing VSVg protein on the surface can assist VLP to transport between cells and infect target cells. The success of infection can be indicated by the expression of GFP.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 3244
Illegal PstI site found at 3946
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2056
Illegal NheI site found at 3316
Illegal PstI site found at 3244
Illegal PstI site found at 3946
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2439
Illegal BamHI site found at 2676
Illegal BamHI site found at 3359
Illegal BamHI site found at 4594
Illegal XhoI site found at 1836
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 3244
Illegal PstI site found at 3946
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 3244
Illegal PstI site found at 3946
Illegal NgoMIV site found at 1647
Illegal AgeI site found at 2575
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 4638
Illegal BsaI.rc site found at 4478
Illegal SapI.rc site found at 4701

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 A novel SEND system based on BBa_K4140013. VLP formed by PEG10 can carry GFP mRNA, and vesicles containing VSVg protein on the surface can assist VLP to transport between cells and infect target cells. The success of infection can be indicated by the expression of GFP.
+===Sequence and Features===
 <!-- Add more about the biology of this part here
@@ Line 9: / Line 11: @@
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-<span class='h3bb'>Sequence and Features</span>
+<span class='h3bb'></span>
 <partinfo>BBa_K5301025 SequenceAndFeatures</partinfo>