Difference between revisions of "Part:BBa K5236015"
Line 19: Line 19:
  
 
<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95f-sequnce-cycle-3.png" width = "50%"><br></html></center>
 
<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/k95f-sequnce-cycle-3.png" width = "50%"><br></html></center>
−
<center>Fig.1 The result of DNA sequencing  </center>
+
<center>Fig.1 The result of pET28a-pelB-IsPETase(K95S)-6xHis DNA sequencing  </center>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 09:03, 1 October 2024


pET28a-pelB-IsPETase(K95S)-6xHis

This composite part codes for the mutated IsPETase K95S and expresses the enzyme in the extracellular space of chassis.

T7 promoter:used to regulate gene expression of recombinantproteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome. IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.


Fig.1 The result of pET28a-pelB-IsPETase(K95S)-6xHis DNA sequencing

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005