Difference between revisions of "Part:BBa K5301029"

Revision as of 09:01, 1 October 2024

spNW15 expression pathway (T7)

This pathway expresses the SpyCatcher-NW15-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW15 to construct nanodiscs.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 496
Illegal SpeI site found at 448
Illegal PstI site found at 664
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 496
Illegal NheI site found at 64
Illegal SpeI site found at 448
Illegal PstI site found at 664
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 496
Illegal BglII site found at 552
Illegal BglII site found at 924
Illegal BamHI site found at 97
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 496
Illegal SpeI site found at 448
Illegal PstI site found at 664
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 496
Illegal SpeI site found at 448
Illegal PstI site found at 664
Illegal AgeI site found at 1027
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+We used this pathway to express spNW15 by using T7 expression system in <i>E.coli</i>, and the expression of spNW15 can be induced by IPTG. For more information on spNW15, go to <partinfo>BBa_K5301013</partinfo>.
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K5301029 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K5301013 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K5301029 parameters</partinfo>
+<partinfo>BBa_K5301013 parameters</partinfo>
 <!-- -->