Difference between revisions of "Part:BBa K5384001"
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<partinfo>BBa_K5384001 short</partinfo> | <partinfo>BBa_K5384001 short</partinfo> | ||
| − | + | Vglycin contains six cysteine residues at sites 3,7,15,20,22 and 32 and forms three pairs of disulfide bonds. It can resist the degradation of gastrointestinal protease, with oral hypoglycemic effect[1,2]. | |
<html> | <html> | ||
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.image-box { | .image-box { | ||
text-align: center; | text-align: center; | ||
| − | + | margin: 0 auto; | |
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padding: 10px; | padding: 10px; | ||
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display: flex; | display: flex; | ||
flex-direction: column; | flex-direction: column; | ||
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.image-name { | .image-name { | ||
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margin-top: 8px; | margin-top: 8px; | ||
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</style> | </style> | ||
| − | <div class="image-box"> | + | <div class="image-box" style="width: 350px;"> |
<div class="image-container"> | <div class="image-container"> | ||
<img src="https://static.igem.wiki/teams/5384/part-ku/part-1.jpg"> | <img src="https://static.igem.wiki/teams/5384/part-ku/part-1.jpg"> | ||
</div> | </div> | ||
| − | <div class="image-name">Figure 1 | + | <div class="image-name">Figure 1 The three-dimensional structure of Vglycin</div> |
</div> | </div> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | The concrete biological functions of | + | The concrete biological functions of Vg have been proved by previous studies.The long-term administration of Vg has excellent hypoglycemic and lipid-lowering effect in mice. P37 has a hypoglycemic effect comparable to metformin on (Figure 2). |
| − | Moreover, previous studies also proved | + | Moreover, previous studies also proved Vg can repaired the damage pancreas induced by streptozotocin(Figure 3). |
| − | + | ||
<html> | <html> | ||
<div class="image-box"> | <div class="image-box"> | ||
<div class="two-images-container"> | <div class="two-images-container"> | ||
| − | <div class="image-container"> | + | <div class="image-container" style="max-width: 50%;"> |
| − | <img src="https://static.igem.wiki/teams/5384/part-ku/ | + | <img src="https://static.igem.wiki/teams/5384/part-ku/part2-2.png"> |
| − | <div class="image-name">Figure 2 | + | <div class="image-name">Figure 2 Vglycin normalized fasting plasma glucose levels in HFD-fed C57BL/6J mice. On days 0, 35, 70, 105, 140, 175, fasting blood glucose of these mice was measured.</div> |
</div> | </div> | ||
| − | <div class="image-container"> | + | <div class="image-container" style="max-width: 50%;"> |
<img src="https://static.igem.wiki/teams/5384/part-ku/part-3.jpg"> | <img src="https://static.igem.wiki/teams/5384/part-ku/part-3.jpg"> | ||
| − | <div class="image-name">Figure 3 | + | <div class="image-name">Figure 3 H&E staining of the pancreatic sections.</div> |
</div> | </div> | ||
</div> | </div> | ||
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===Application=== | ===Application=== | ||
| − | Compared with traditional small-molecule chemical drugs, active peptide drugs P37 have small molecular weight, simple structure and no immunogenicity. The mechanism of action is clear and the side effects are low; Synthetic purity, easy synthesis and other unique advantages[3].It can resist the degradation of gastrointestinal proteases and has the effect of oral hypoglycemia. | + | Compared with traditional small-molecule chemical drugs, active peptide drugs P37 have small molecular weight, simple structure and no immunogenicity. The mechanism of action is clear and the side effects are low; Synthetic purity, easy synthesis and other unique advantages[3]. It can resist the degradation of gastrointestinal proteases and has the effect of oral hypoglycemia. |
===Functional Parameters=== | ===Functional Parameters=== | ||
| Line 75: | Line 73: | ||
===References=== | ===References=== | ||
| − | 1 | + | [1]Younossi, Z. M., Koenig, A. B., Abdelatif, D., Fazel, Y., Henry, L., & Wymer, M. (2016).Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology, 64(1), 73–84. |
| − | 2 | + | |
| − | 3 | + | [2]Neuschwander-Tetri, B. A. (2017). Non-alcoholic fatty liver disease. BMC Med. 15(1), 45.Charlton, M. (2004). Nonalcoholic fatty liver disease: A review of current understandingand future impact. Clin gastroenterol hepatol. 2, 1048–1058. |
| + | |||
| + | [3]Vuppalanchi, R., & Chalasani, N. (2009). Nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: Selected practical issues in their evaluation and management.Hepatology, 49, 306–317. | ||
Latest revision as of 08:56, 1 October 2024
Vglycin (Vg)
Vglycin contains six cysteine residues at sites 3,7,15,20,22 and 32 and forms three pairs of disulfide bonds. It can resist the degradation of gastrointestinal protease, with oral hypoglycemic effect[1,2].
Figure 1 The three-dimensional structure of Vglycin
Usage and Biology
The concrete biological functions of Vg have been proved by previous studies.The long-term administration of Vg has excellent hypoglycemic and lipid-lowering effect in mice. P37 has a hypoglycemic effect comparable to metformin on (Figure 2). Moreover, previous studies also proved Vg can repaired the damage pancreas induced by streptozotocin(Figure 3).
Figure 2 Vglycin normalized fasting plasma glucose levels in HFD-fed C57BL/6J mice. On days 0, 35, 70, 105, 140, 175, fasting blood glucose of these mice was measured.
Figure 3 H&E staining of the pancreatic sections.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Application
Compared with traditional small-molecule chemical drugs, active peptide drugs P37 have small molecular weight, simple structure and no immunogenicity. The mechanism of action is clear and the side effects are low; Synthetic purity, easy synthesis and other unique advantages[3]. It can resist the degradation of gastrointestinal proteases and has the effect of oral hypoglycemia.
Functional Parameters
| function | This sequence codes for Vglycin, which is a peptide derived from leguminous plants. It contains 37 amino acids. It has six cysteine residues located at positions 3, 7, 15, 20, 22, and 32, forming three pairs of disulfide bonds within the molecule. It is resistant to degradation by gastrointestinal proteases and has the effect of lowering blood sugar when taken orally. Vg has excellent hypoglycemic effect on mice and can repair damaged pancreas. |
References
[1]Younossi, Z. M., Koenig, A. B., Abdelatif, D., Fazel, Y., Henry, L., & Wymer, M. (2016).Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology, 64(1), 73–84.
[2]Neuschwander-Tetri, B. A. (2017). Non-alcoholic fatty liver disease. BMC Med. 15(1), 45.Charlton, M. (2004). Nonalcoholic fatty liver disease: A review of current understandingand future impact. Clin gastroenterol hepatol. 2, 1048–1058.
[3]Vuppalanchi, R., & Chalasani, N. (2009). Nonalcoholic fatty liver disease and nonalcoholic steatohepatitis: Selected practical issues in their evaluation and management.Hepatology, 49, 306–317.