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| − | __NOTOC__
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| − | <partinfo>BBa_K5143028 short</partinfo>
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| − | <meta charset="UTF-8">
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| − | <meta name="viewport" content="width=device-width, initial-scale=1.0">
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| − | <title>Protein Description</title>
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| − | <h1>Description</h1>
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| − | Our team has nicknamed this plasmid “<b>Bt toxin plasmid</b>”. The final part pUC57-5'URA-GAP_promoter-alphafactorV1-plu1537-dCBD-TDH1_term-3'URA is composed of the plu1537 gene is a antitermite toxin (known as Bt toxin) fused to a dCBD and an alphafactor signal. <br> This final construct has been transformed in the BY4741 <i> S. cerevisiae </i> strain. This transformed yeast will help us to functionalize bacterial cellulose with antitermite properties by a coculture with the yeast and the cellulose producing bacteria <i> Komagataeibacter rhaeticus </i>.
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| − | <div class="image-container">
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| − | <figure>
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| − | <img src="https://static.igem.wiki/teams/5143/bba-k5143016-bt-toxin-cbd.png" width="700" alt="Plu1537">
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| − | <figcaption>Figure 1: Production of the fused plu1537</figcaption>
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| − | <h1>Construction</h1>
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| − | <p>
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| − | pUC57-5'URA-GAP_promoter-alphafactorV1-plu1537-dCBD-TDH1_term-3'URA is composed of the pUC57 backbone <a href="https://parts.igem.org/Part:BBa_K5143005" target="_blank">BBa_K5143005</a> and the alphafactor-plu1537-dCBD composite part <a href="https://parts.igem.org/Part:BBa_K5143016" target="_blank">BBa_K5143016</a>. <br>
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| − | The alphafactor-plu1537-dCBD composite part was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>.
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| − | <h1>References</h1>
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| − | <p>
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| − | 1. Linder, M.; Salovuori, I.; Ruohonen, L.; Teeri, T.T., 1996. Characterization of a Double Cellulose-binding Domain. SYNERGISTIC HIGH AFFINITY BINDING TO CRYSTALLINE CELLULOSE. Journal of Biological Chemistry, 271(35), pp.21268–21272. Available at: http://www.jbc.org/content/271/35/21268.full <br>
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| − | 2. Gilbert, C.; Tang, T.-C.; Ott, W.; Dorr, B. A.; Shaw, W. M.; Sun, G. L.; Lu, T. K.; Ellis, T. Living Materials with Programmable Functionalities Grown from Engineered Microbial Co-Cultures. Nat. Mater. 2021, 20 (5), 691–700. https://doi.org/10.1038/s41563-020-00857-5. <br>
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| − | 3. M. S. Kelker et al., PLOS ONE 9, (2014).
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| − | <!-- Add more about the biology of this part here
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| − | ===Usage and Biology===
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| − | <h1>Sequence and Features</h1>
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| − | <partinfo>BBa_K5143028 SequenceAndFeatures</partinfo>
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| − | <!-- Uncomment this to enable Functional Parameter display
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| − | ===Functional Parameters===
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| − | <partinfo>BBa_K5143028 parameters</partinfo>
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