Difference between revisions of "Part:BBa K218018:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K218018 short</partinfo>
 
<partinfo>BBa_K218018 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Emily!
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The plasmid containing LuxOD47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999).
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>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E).
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===Source===
 
===Source===
  
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Bleh
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Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.
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===References===
 
===References===

Revision as of 02:23, 22 October 2009

LuxO D47E with strong RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 136
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 824
    Illegal SapI.rc site found at 786


Design Notes

The plasmid containing LuxOD47E (pJAF822) was obtained from Bassler lab (Freeman and Bassler, 1999).

>gi|156972381:c2983472-2982069 Vibrio harveyi ATCC BAA-1116 chromosome I, complete sequence Nucleotide number 141 has been mutated from T to G, thus bases 139-141 have been changed from GAT (Aspartic acid, D) to GAG (Glutamine, E).



Source

Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.


References