Difference between revisions of "Part:BBa K243014"
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<partinfo>BBa_K243014 short</partinfo>
 
<partinfo>BBa_K243014 short</partinfo>
  
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This part unites some of our parts. The combination of a Histidin-Tag, a Lipocalin-Tag and the linked protein domain Fok_a makes it possible to purify and detect the function of the protein in one step. The fluoresceinA tag allows the measurement by quenching and the coupling to an anticalin linked oligo.
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This combination uses the benefits of an His-tag for purification. It is also linked with a FluroescineA-tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee the independent function of FluA tag and the protein domain Fok_i.
  
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===Usage and Biology===
 
===Usage and Biology===
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This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243017 to built to a functional heterodimer. The FluA tag leads the part to DNA which is hybridized with an Fluorescein labeled oligonucleotide. The middle linker makes a distance from 24bp between the FluA and the linked Fok_i protein domain. The His-tag serves as purification tag for a Ni-NTA column purification.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K243014 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243014 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:18, 22 October 2009

His-FluA-Middle Linker-Fok_i

This combination uses the benefits of an His-tag for purification. It is also linked with a FluroescineA-tag. The Middle Linker (GlySerGlyGly)x2 connects the parts and adds additional space between them to guarantee the independent function of FluA tag and the protein domain Fok_i.

Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243017 to built to a functional heterodimer. The FluA tag leads the part to DNA which is hybridized with an Fluorescein labeled oligonucleotide. The middle linker makes a distance from 24bp between the FluA and the linked Fok_i protein domain. The His-tag serves as purification tag for a Ni-NTA column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 272
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]