Difference between revisions of "Part:BBa K5291042"
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===Usage and Biology===
 
===Usage and Biology===
  
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In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.<br>
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<html><img width = "700" src="https://static.igem.wiki/teams/5291/images/part-wyn/cyp-plasmid.png" /></html><br>
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<b>Fig.1 The map of plasmid pAB1-CYPY96F-VHB.</b><br><br>
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Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.<br>
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<html><img width = "500" src="https://static.igem.wiki/teams/5291/images/part-wyn/sds-page-cyp.png" /></html><br>
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<b>Fig.2 The SDS-PAGE result of CYPY96F.</b><br><br>
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Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.<br>
  
 
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Revision as of 08:13, 1 October 2024


CYPY96F

CYPY96F: P450cam mutant with higher alkane oxidation activity.

Usage and Biology

In degradation module we constructed pAB1-CYPY96F-VHB to ensure the expression of emzymes related to degradation.

Fig.1 The map of plasmid pAB1-CYPY96F-VHB.

Moreover,CYPY96F proteins was successfully expressed in the strains. SDS-PAGE was performed and the following results were obtained.

Fig.2 The SDS-PAGE result of CYPY96F.

Since we set CYPY96F and VHB as a whole, the overall functions need moving to the part BBa_K5291034.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 312
    Illegal BamHI site found at 392
    Illegal BamHI site found at 1221
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 109
    Illegal NgoMIV site found at 277
  • 1000
    COMPATIBLE WITH RFC[1000]