Difference between revisions of "Part:BBa K243015"

 
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<partinfo>BBa_K243015 short</partinfo>
 
<partinfo>BBa_K243015 short</partinfo>
  
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These part units some of our parts and their features.
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This combination uses the benefits of a Streptavidin-tag for purification. It is also linked with a DigoxigeninA tag [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243003 DigA]. The Split Linker connects the parts and adds additional space between them to guarantee the independent function of DigA tag and the protein domain Fok_i.
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===Usage and Biology===
 
===Usage and Biology===
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This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243024 to build a functional heterodimer. The DigA tag guides the part to DNA which is hybridized with a Digoxigenin labeled oligonucleotide. The Split Linker creates a distance of 51bp between the DigA and the linked Fok_i protein domain. The Strep Tag serves as a purification tag for Streptavidin column purification.
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K243015 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243015 SequenceAndFeatures</partinfo>
  

Latest revision as of 02:15, 22 October 2009

Strep-DigA-Split Linker-Fok_i

This combination uses the benefits of a Streptavidin-tag for purification. It is also linked with a DigoxigeninA tag DigA. The Split Linker connects the parts and adds additional space between them to guarantee the independent function of DigA tag and the protein domain Fok_i.


Usage and Biology

This composite part is one part of our universal endonuclease and it needs another composite part like BBa_K243024 to build a functional heterodimer. The DigA tag guides the part to DNA which is hybridized with a Digoxigenin labeled oligonucleotide. The Split Linker creates a distance of 51bp between the DigA and the linked Fok_i protein domain. The Strep Tag serves as a purification tag for Streptavidin column purification.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]