Difference between revisions of "Part:BBa K192000"
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=Encapsulin= | =Encapsulin= | ||
| − | BBa_K192000 | + | BBa_K192000 is a shell-forming protein known as "encapsulin", which we derived from the ''TM0785'' plasmid in ''T. maritima''. The protein is a monomer which assembles into a thin icosahedral shell with a diameter of 240 angstroms resulting in a nanocompartment lined with conserved binding sites for short polypeptide tags present as C-terminal extensions of enzymes. In ''T. maritima'', these enzymes are involved in oxidative-stress response. We have isolated the encapsulin protein in ''E. coli'', in which we plan to characterize as an expression system for the application of channeling candidate enzyme pairs identified ''in silico''. |
Revision as of 02:03, 22 October 2009
Encapsulin
BBa_K192000 is a shell-forming protein known as "encapsulin", which we derived from the TM0785 plasmid in T. maritima. The protein is a monomer which assembles into a thin icosahedral shell with a diameter of 240 angstroms resulting in a nanocompartment lined with conserved binding sites for short polypeptide tags present as C-terminal extensions of enzymes. In T. maritima, these enzymes are involved in oxidative-stress response. We have isolated the encapsulin protein in E. coli, in which we plan to characterize as an expression system for the application of channeling candidate enzyme pairs identified in silico.