Difference between revisions of "Part:BBa K5276004"
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Bacteriophages initially used recombination direction factors (RDF) to cut their own genes out of host DNA to form progeny phages. RDF can control the activity of recombinases to promote specific recombination reaction directions. Research indicates that the Gp44 protein(Mandali et al.,2017), as a recombination direction factor, can enhance the excision or inversion reaction of A118 between attL and attR sites. | Bacteriophages initially used recombination direction factors (RDF) to cut their own genes out of host DNA to form progeny phages. RDF can control the activity of recombinases to promote specific recombination reaction directions. Research indicates that the Gp44 protein(Mandali et al.,2017), as a recombination direction factor, can enhance the excision or inversion reaction of A118 between attL and attR sites. | ||
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| + | For details about the specific principles of A118 recombinase and RDF, please refer to A118 recombinase (BBa_K5276001). | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Latest revision as of 07:14, 1 October 2024
Gp44
Bacteriophages initially used recombination direction factors (RDF) to cut their own genes out of host DNA to form progeny phages. RDF can control the activity of recombinases to promote specific recombination reaction directions. Research indicates that the Gp44 protein(Mandali et al.,2017), as a recombination direction factor, can enhance the excision or inversion reaction of A118 between attL and attR sites.
For details about the specific principles of A118 recombinase and RDF, please refer to A118 recombinase (BBa_K5276001).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 22
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]