Difference between revisions of "Part:BBa K5382124:Design"

 
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<partinfo>BBa_K5382124 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5382124 SequenceAndFeatures</partinfo>
  
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===Design Notes===
 
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CL7, an engineered variant of colicin CE7, is a protein tag obtained by engineering CE7 to remove its DNA-binding and catalytic activity, preserving its high affinity binding ability to the corresponding inhibitory protein Im7
 
CL7, an engineered variant of colicin CE7, is a protein tag obtained by engineering CE7 to remove its DNA-binding and catalytic activity, preserving its high affinity binding ability to the corresponding inhibitory protein Im7
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===References===
 

Revision as of 06:06, 1 October 2024


CL7-protein tag


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 133
    Illegal BglII site found at 81
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 133
    Illegal AgeI site found at 31
    Illegal AgeI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]



Source

CL7, an engineered variant of colicin CE7, is a protein tag obtained by engineering CE7 to remove its DNA-binding and catalytic activity, preserving its high affinity binding ability to the corresponding inhibitory protein Im7