Difference between revisions of "Part:BBa K5291030"

 
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<partinfo>BBa_K5291030 short</partinfo>
 
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We use this composite part to improve the efficiency of bicarbonate-C02 conversion in Rhodopseudomonas palustris CGA009, speeding up the process of carbon fixation.
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We use this composite part to improve the efficiency of bicarbonate-CO<sub>2</sub> conversion in <i>Rhodopseudomonas palustris</i> CGA009, speeding up the process of carbon fixation.
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The gene <i>acaP</i> in <i>Rhodopseudomonas palustris</i> is able to encode a kind of α-CAs that can speed up the conversion between CO<sub>2</sub> and bicarbonate. By the method of <i>acaP</i> high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO<sub>2</sub> conversion in <i>R. palustris</i> increase and the engineered bacteria is able to take advantage of carbon to produce cellulose. We make use of the promoter pT<sub>7</sub> to realize the constitutive expression of <i>acaP</i>. And BiTerm, the terminator which can come into effect in our engineered bacteria, is used in the system.<br><br>
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<html><img width = "550" src="https://static.igem.wiki/teams/5291/images/part-cr/acap-schema.png" /></html><br>
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<b>Fig.1 The schema of pS-<i>acaP</i>-BiTerm.</b><br><br>
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And we also successfully construct the strain with the complete plasmid.<br><br>
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<html><img width = "300" src="https://static.igem.wiki/teams/5291/images/part-cr/acap-colony-pcr.png" /></html>
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<html><img width = "630" src="https://static.igem.wiki/teams/5291/images/part-cr/4-pbbr1mcs2-r-ca-map.png" /></html><br>
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<b>Fig.2 The AGE figure of pBBR1MCS2-pS-<i>acaP</i> and the map of the plasmid.</b><br><br>
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It is a great pity that we have not finished the verification of the function due to rush time.<br><br>
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Latest revision as of 05:34, 1 October 2024


pBBRMCS2-acaP

We use this composite part to improve the efficiency of bicarbonate-CO2 conversion in Rhodopseudomonas palustris CGA009, speeding up the process of carbon fixation.

Usage and Biology

The gene acaP in Rhodopseudomonas palustris is able to encode a kind of α-CAs that can speed up the conversion between CO2 and bicarbonate. By the method of acaP high copies, the rate of bicarbonate intake as well as the speed of bicarbonate- CO2 conversion in R. palustris increase and the engineered bacteria is able to take advantage of carbon to produce cellulose. We make use of the promoter pT7 to realize the constitutive expression of acaP. And BiTerm, the terminator which can come into effect in our engineered bacteria, is used in the system.


Fig.1 The schema of pS-acaP-BiTerm.

And we also successfully construct the strain with the complete plasmid.


Fig.2 The AGE figure of pBBR1MCS2-pS-acaP and the map of the plasmid.

It is a great pity that we have not finished the verification of the function due to rush time.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 242
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 242
    Illegal NotI site found at 845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 171
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 242
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 242
  • 1000
    COMPATIBLE WITH RFC[1000]