Difference between revisions of "Part:BBa K5325000:Design"

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(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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Addition of an N-terminal PelB signal sequence for the secretion of the protein into the periplasm via the Sec system; FLAG tag helps with protein purification; codon optimized for expression in E. coli
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Addition of a FLAG tag that aids protein purification and visualization on Western Blot.<br>
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+
DNA sequence was codon optimized for expression in <i>E. coli</i>, which included the change in the start codon from GTG to ATG.
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===Source===
 
===Source===

Revision as of 04:09, 1 October 2024


ABO_plaA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 757
    Illegal AgeI site found at 673
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Addition of a FLAG tag that aids protein purification and visualization on Western Blot.
DNA sequence was codon optimized for expression in E. coli, which included the change in the start codon from GTG to ATG.

Source

The sequence was taken from Alcanivorax borkumensis genome

References