Difference between revisions of "Part:BBa K5236018"

Revision as of 03:44, 1 October 2024

pET28a-pelB-IsPETase(M154L)-6xHis

This composite part codes for the mutated IsPETase M154L and expresses the enzyme in the extracellular space of chassis.

T7 promoter: used to regulate gene expression of recombinant proteins.

Lac operator: used to control gene expression by lactose.

RBS: the location to bind ribosome.

IsPETase M154L: This sequence encodes for a mutated IsPETase M154L and is derived from Escherichia coli.

pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.

6xHis: a tag for the purification of protein which focus in the production of recombinant proteins.

T7 terminator: terminates transcription.

Fig.1 The result of DNA sequencing

Sequence and Features

Assembly Compatibility:

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K5236018 short</partinfo>
+This composite part codes for the mutated IsPETase M154L and expresses the enzyme in the extracellular space of chassis.
 T7 promoter: used to regulate gene expression of recombinant proteins.
 Lac operator: used to control gene expression by lactose.
 RBS: the location to bind ribosome.
 IsPETase M154L: This sequence encodes for a mutated IsPETase M154L and is derived from Escherichia coli.
 pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane.
 xHis: a tag for the purification of protein which focus in the production of recombinant proteins.
 T7 terminator: terminates transcription.
+<center><html><img src ="https://static.igem.wiki/teams/5236/part-images/m154l-sequence-cycle-3.png" width = "50%"><br></html></center>
+<center>Fig.1 The result of DNA sequencing  </center>
 <!-- Add more about the biology of this part here