Difference between revisions of "Part:BBa K5115038"
| Line 6: | Line 6: | ||
__TOC__ | __TOC__ | ||
===Introduction=== | ===Introduction=== | ||
| − | This composite part combines [https://parts.igem.org/Part:BBa_K5115035 BBa_K5115035(ribozyme+RBS+MTA+stem-loop)], [https://parts.igem.org/Part:BBa_K5115036 BBa_K5115036(ribozyme+RBS+hpn+stem-loop)]and [https://parts.igem.org/Part:BBa_K5115033 BBa_K5115033(ribozyme+RBS+RcnR_C35L+stem-loop)] . We introduced this ribozyme-assisted polycistronic co-expression system | + | This composite part combines [https://parts.igem.org/Part:BBa_K5115035 BBa_K5115035(ribozyme+RBS+MTA+stem-loop)], [https://parts.igem.org/Part:BBa_K5115036 BBa_K5115036(ribozyme+RBS+hpn+stem-loop)]and [https://parts.igem.org/Part:BBa_K5115033 BBa_K5115033(ribozyme+RBS+RcnR_C35L+stem-loop)] . We introduced this ribozyme-assisted polycistronic co-expression system from [https://2022.igem.wiki/fudan/parts 2022]. By inserting [https://parts.igem.org/Part:BBa_K4765020 ribozyme sequences] between CDSs in a polycistron, the RNA sequences of Twister ribozyme conduct self-cleaving, and the polycistronic mRNA transcript is thus co-transcriptionally converted into individual mono-cistrons ''in vivo''. |
With this design, we achieve co-expression of [https://parts.igem.org/Part:BBa_K5115050 MTA], [https://parts.igem.org/Part:BBa_K1151001 hpn], [https://parts.igem.org/Part:BBa_K5115000 RcnR C35L] at similar level. MTA is a protein that can bind with nickel ions to reduce its toxity to the ''E.coli''. The Hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the ''E.coli''. RcnR C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate. | With this design, we achieve co-expression of [https://parts.igem.org/Part:BBa_K5115050 MTA], [https://parts.igem.org/Part:BBa_K1151001 hpn], [https://parts.igem.org/Part:BBa_K5115000 RcnR C35L] at similar level. MTA is a protein that can bind with nickel ions to reduce its toxity to the ''E.coli''. The Hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the ''E.coli''. RcnR C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate. | ||
| + | ===Usage and Biology=== | ||
| + | This part is eventually chosen as a component of [https://parts.igem.org/Part:BBa_K5115068 mineral nickel module], tuning the nickel ion transport rate and reducing nickel's toxity to the ''E.coli''. | ||
| + | ===Characterization=== | ||
| + | ====Growth curve of ''E.coli''=== | ||
<!-- --> | <!-- --> | ||
Revision as of 03:03, 1 October 2024
ribozyme connected: MTA, Hpn, RcnR_C35L
Contents
Introduction
This composite part combines BBa_K5115035(ribozyme+RBS+MTA+stem-loop), BBa_K5115036(ribozyme+RBS+hpn+stem-loop)and BBa_K5115033(ribozyme+RBS+RcnR_C35L+stem-loop) . We introduced this ribozyme-assisted polycistronic co-expression system from 2022. By inserting ribozyme sequences between CDSs in a polycistron, the RNA sequences of Twister ribozyme conduct self-cleaving, and the polycistronic mRNA transcript is thus co-transcriptionally converted into individual mono-cistrons in vivo.
With this design, we achieve co-expression of MTA, hpn, RcnR C35L at similar level. MTA is a protein that can bind with nickel ions to reduce its toxity to the E.coli. The Hpn is a protein that can sequester metals that accumulate internally to reduce nickel's toxity to the E.coli. RcnR C35L can regulate the nickel ion channel proteins in the cell membrane to tune the nickel ion transport rate.
Usage and Biology
This part is eventually chosen as a component of mineral nickel module, tuning the nickel ion transport rate and reducing nickel's toxity to the E.coli.
Characterization
=Growth curve of E.coli
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 935
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 198
- 1000COMPATIBLE WITH RFC[1000]