Difference between revisions of "Part:BBa K5301026"
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<partinfo>BBa_K5301026 short</partinfo> | <partinfo>BBa_K5301026 short</partinfo> | ||
| − | This pathway expresses | + | This pathway expresses sGFP1-10 tether (BBa_K5301017) using T7 expression system，containing T7 promoter, mSA, sGFP1-10, and T7 terminator. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | We used this pathway to express sGFP1-10 tether（mSA - sGFP1-10） by using T7 expression system in <i>E.coli</i>, and the expression of sGFP1-10 tether can be induced by IPTG. For more information on sGFP1-10 tether[1-10], go to <partinfo>BBa_K5301017</partinfo>. | ||
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Revision as of 02:28, 1 October 2024
sGFP1-10 tether expression pathway (T7)
This pathway expresses sGFP1-10 tether (BBa_K5301017) using T7 expression system，containing T7 promoter, mSA, sGFP1-10, and T7 terminator.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 159
Illegal AgeI site found at 219 - 1000COMPATIBLE WITH RFC[1000]