Difference between revisions of "Part:BBa K5079013"
 
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<partinfo>BBa_K5079013 short</partinfo>
 
<partinfo>BBa_K5079013 short</partinfo>
  
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This is the forward primer for wild type molecular switch 9. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.
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This part is the forward primer for switch 7 (K45_CP_P95, part BBa_K5079032) into pET-28a. It allows for the addition of overhangs to the sequence of protein switch 7 that are complementary to the pET-28a sequence for cloning. It has a melting point of 65.0°C . Part "BBa_ K5079014" used as reverse primer. Cloned in frame with a His Tag. Used with q5 polymerase mastermix.
  
 
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Latest revision as of 01:51, 1 October 2024


K45_CP_P95 Switch primers into pET-28a Switch 7 (Fwd)

This part is the forward primer for switch 7 (K45_CP_P95, part BBa_K5079032) into pET-28a. It allows for the addition of overhangs to the sequence of protein switch 7 that are complementary to the pET-28a sequence for cloning. It has a melting point of 65.0°C . Part "BBa_ K5079014" used as reverse primer. Cloned in frame with a His Tag. Used with q5 polymerase mastermix.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]