Difference between revisions of "Part:BBa K5079013"

Revision as of 01:28, 1 October 2024

K45_CP_P95 Switch primers into pET-28a Switch 7 (Fwd)

This is the forward primer for wild type molecular switch 9. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K5079013 short</partinfo>
-This is the forward primer for Part:BBa_K5079032. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. The sequence is: ACTTTAAGAAGGAGATATAC atgtcgggaaatcccttgg. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.
+This is the forward primer for wild type molecular switch 9. This is cloned in frame with the his tag, primers were used with the q5 polymerase master mix. 3’ GC Content is 53%, 3' length is 19nt and 3' Tm is 65°C.
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