Difference between revisions of "Part:BBa K5439006"

Revision as of 23:52, 30 September 2024

FRET-based system for the detection of ibuprofen

FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.(BBa_K5439005),an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP(BBa_K1159302)as energy donor and mVenus(BBa_K1907000)as an energy acceptor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 2306
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1177
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2174
Illegal BsaI.rc site found at 2959

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 <partinfo>BBa_K5439006 short</partinfo>
-FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.[https://parts.igem.org/Part:BBa_K5439005 (BBa_K5439005)], an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP[https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as energy donor and mVenus [https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor.
+FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.[https://parts.igem.org/Part:BBa_K5439005 (BBa_K5439005)],an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP[https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)]as energy donor and mVenus[https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)]as an energy acceptor.
 <!-- Add more about the biology of this part here