Difference between revisions of "Part:BBa K5439006"

 
Line 3: Line 3:
 
<partinfo>BBa_K5439006 short</partinfo>
 
<partinfo>BBa_K5439006 short</partinfo>
  
−
FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.  (BBa_K5439005), an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP (BBa_K1159302) as energy donor and mVenus (BBa_K1907000) as an energy acceptor.  
+
FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.  (BBa_K5439005), an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP [https://parts.igem.org/Part:BBa_K1159302 (BBa_K1159302)] as energy donor and mVenus [https://parts.igem.org/Part:BBa_K1907000 (BBa_K1907000)] as an energy acceptor.  
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 23:50, 30 September 2024


FRET-based system for the detection of ibuprofen

FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp. (BBa_K5439005), an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP (BBa_K1159302) as energy donor and mVenus (BBa_K1907000) as an energy acceptor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2306
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2174
    Illegal BsaI.rc site found at 2959