Difference between revisions of "Part:BBa K243033"
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This is our expression vector. The plasmid has the restriction sites XbaI NgoMIV_part_AgeI_SpeI_NotI_PstI
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This is our expression vector. The plasmid has the restriction sites XbaI NgoMIV_part_AgeI_SpeI_NotI_PstI.
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[[Image:Freiburg09 pEx.JPG]]
 
[[Image:Freiburg09 pEx.JPG]]
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===Usage and Biology===
 
===Usage and Biology===
 
We used this vector containing a resistance for ampicillin for the expression of the His-FluA-Split-Fok_i (BBa_K243010)construct. The expression of the other heterodimeric Fok construct His-Dig-Split-Fok_a (BBa_K243036)  took place in a different vector pJS419 with chloramphenicol resistance. This enables a positive selection of the bacteria strain XL1 blue cotransformed with both plasmids.
 
We used this vector containing a resistance for ampicillin for the expression of the His-FluA-Split-Fok_i (BBa_K243010)construct. The expression of the other heterodimeric Fok construct His-Dig-Split-Fok_a (BBa_K243036)  took place in a different vector pJS419 with chloramphenicol resistance. This enables a positive selection of the bacteria strain XL1 blue cotransformed with both plasmids.
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K243033 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K243033 SequenceAndFeatures</partinfo>

Revision as of 00:56, 22 October 2009

pEX


This is our expression vector. The plasmid has the restriction sites XbaI NgoMIV_part_AgeI_SpeI_NotI_PstI.


Freiburg09 pEx.JPG


Usage and Biology

We used this vector containing a resistance for ampicillin for the expression of the His-FluA-Split-Fok_i (BBa_K243010)construct. The expression of the other heterodimeric Fok construct His-Dig-Split-Fok_a (BBa_K243036) took place in a different vector pJS419 with chloramphenicol resistance. This enables a positive selection of the bacteria strain XL1 blue cotransformed with both plasmids.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2734
    Illegal XbaI site found at 1523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2735
    Illegal PstI site found at 2749
    Illegal NotI site found at 2742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1808
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2735
    Illegal XbaI site found at 1523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2725
    Illegal XbaI site found at 1523
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3800
    Illegal SapI.rc site found at 4799