Difference between revisions of "Part:BBa K5102060:Design"

 
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===References===
 
===References===
 +
Cox, D. B. T. et al. RNA editing with CRISPR-Cas13. Science 358, 1019–1027 (2017).

Latest revision as of 22:04, 30 September 2024


dPspCas13b fusion with ADAR2DD


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 235
    Illegal PstI site found at 631
    Illegal PstI site found at 1021
    Illegal PstI site found at 2443
    Illegal PstI site found at 3277
    Illegal PstI site found at 3930
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2884
    Illegal PstI site found at 235
    Illegal PstI site found at 631
    Illegal PstI site found at 1021
    Illegal PstI site found at 2443
    Illegal PstI site found at 3277
    Illegal PstI site found at 3930
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1787
    Illegal BamHI site found at 839
    Illegal BamHI site found at 3310
    Illegal XhoI site found at 4011
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 235
    Illegal PstI site found at 631
    Illegal PstI site found at 1021
    Illegal PstI site found at 2443
    Illegal PstI site found at 3277
    Illegal PstI site found at 3930
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 235
    Illegal PstI site found at 631
    Illegal PstI site found at 1021
    Illegal PstI site found at 2443
    Illegal PstI site found at 3277
    Illegal PstI site found at 3930
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 2396
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ADAR2DD sequence harbors two amino acid mutations: E488Q and T375G. While E488Q increases the adenosine-to- inosine editing rates, T375G confers increased specificity with slightly reduced activity.


Source

This part was ordered as a synthetic oligo from a DNA synthesis provider.

References

Cox, D. B. T. et al. RNA editing with CRISPR-Cas13. Science 358, 1019–1027 (2017).