Difference between revisions of "Part:BBa K5097009"
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===Usage and Biology===
 
===Usage and Biology===
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This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al., 1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.  
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This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al., 1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.
  
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[[File:T--Suny Oneonta-t7-virus-structure.jpg|200px|frame|center|Figure 1: A labeled visual detailing the various structures of a T7-like phage (Kemp et al., 2005)]]
 
  
  
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[[File:putrescine-pathway.jpg|200px|frame|center|Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)]]
 
  
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:::::::::https://static.igem.wiki/teams/5097/parts/putrescine-pathway.jpg
  
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[[File:https://static.igem.wiki/teams/5097/parts/putrescine-pathway.jpg|caption]]
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:::::::::Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)
  
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===References===
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Shaibe, E et al. “Metabolic pathway for the utilization of L-arginine, L-ornithine, agmatine, and putrescine as nitrogen sources in Escherichia coli K-12.” Journal of bacteriology vol. 163,3 (1985): 933-7. doi:10.1128/jb.163.3.933-937.1985
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Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.
  
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<img src="https://static.igem.wiki/teams/5097/parts/putrescine-pathway.jpg" alt=putrescine-pathway" />
 
  
 
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<partinfo>BBa_K5097009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5097009 SequenceAndFeatures</partinfo>
  
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This gene sequence has been altered to make the SpeA gene RCF10 compatible. To do this, we removed an internal PstI restriction sites, as follows G291A, T1170G, G1434A. These changes will not impact the amino acid sequence.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Latest revision as of 18:10, 30 September 2024


SpeA (arginine decarboxylase)

Usage and Biology

This specific part codes for SpeA gene from E.coli. It expresses the enzyme arginine decarboxylase (Shaibe et al., 1985). This enzyme catalyzes the conversion of L-arginine into agmatine and carbon dioxide, the first step in the metabolic pathway that converts arginine into putrescine (Morris et al., 1969). The 2024 Oneonta iGEM designed this sequence for use in a base producing circuit. This part when co-expressed with SpeB (BBa_K0597010) will construct this pathway and produce putrescine from arginine.



putrescine-pathway.jpg
Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 3 (Charlier et al., 2019)

References

Shaibe, E et al. “Metabolic pathway for the utilization of L-arginine, L-ornithine, agmatine, and putrescine as nitrogen sources in Escherichia coli K-12.” Journal of bacteriology vol. 163,3 (1985): 933-7. doi:10.1128/jb.163.3.933-937.1985

Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1389
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1167
  • 1000
    COMPATIBLE WITH RFC[1000]

This gene sequence has been altered to make the SpeA gene RCF10 compatible. To do this, we removed an internal PstI restriction sites, as follows G291A, T1170G, G1434A. These changes will not impact the amino acid sequence.