Difference between revisions of "Part:BBa K5097012"
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This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars. | This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars. | ||
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| + | :::::::::Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom). Alignment generated using https://www.genome.jp/tools-bin/clustalw. | ||
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Latest revision as of 17:53, 30 September 2024
RBS BBa_B0034 with spacer nucleotides
Usage and Biology
This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars.
- Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom). Alignment generated using https://www.genome.jp/tools-bin/clustalw.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]