Difference between revisions of "Part:BBa K5097012"

Latest revision as of 17:53, 30 September 2024

RBS BBa_B0034 with spacer nucleotides

Usage and Biology

This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars.

Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom). Alignment generated using https://www.genome.jp/tools-bin/clustalw.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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-RBS B0034 with added spacer sequencers
 __NOTOC__
 <partinfo>BBa_K5097012 short</partinfo>
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 This part is a modified form of the Anderson Collections RBS B0034. Additional nucleotides have been added to the 5’ and 3’ ends of the RBS, the sequence of which is AAGAGGAGA. The purpose of these additional nucleotides is to mimic the spacing created by the RCF10 assembly scars.
-[[File:bba-k5097012-figure1.webp|Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom).  Alignment generated using link=https://www.genome.jp/tools-bin/clustalw.]]
+:::::::::https://static.igem.wiki/teams/5097/parts/k012-f1.jpg
+:::::::::Figure 1: Alignment of RBS BBa_B0034 (top) with BBa_B0034 (bottom). Alignment generated using https://www.genome.jp/tools-bin/clustalw.
 <!-- Add more about the biology of this part here
 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K5097013 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K5097012 SequenceAndFeatures</partinfo>