Difference between revisions of "Part:BBa K5136226"

(→‎MT3 and MT2A)
Line 11: Line 11:
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
−
 
+
After deinking, heavy metal ions in the ink will be released into the wastewater. Thus, this wastewater needs harmless treatmentremove before discharging. Hence, we use MTs to treat wastewater to remove heavy metals. And, in order to increase the contact area of MTsand metal ions to improve the adsorption efficiency, we introduce INPNC for surface display of MTs. Thus, this composite part BBa_K5136226 was constructed to express the fuesed protein INPNC-linker-MT3-linker-MT2A which anchored on the surface of bacteria.
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 16:53, 30 September 2024


I0500-B0034-inpnc-linker-mt3-linker-mt2a-B0015

Biology

INPNC

Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).

MT3 and MT2A

The metallothioneins (MTs) are a class of low molecular weight and cysteine-rich metal binding proteins, and each one of them can bind to 6-9 heavy metal ions. The MTs are expressed as intracellular protein and are primarily responsible for metal regulation in cells of living organisms. General MTs can widely non-covalently bind divalent heavy metal ions, such as Zn2+, N2+, Pb2+, Hg2+, Cd2+, as well as As3+, but their effectiveness in treating Cr2O72- is not satisfactory. MT2a and MT3 are metallothioneins(MTs) found in Homo sapiens. MT2A not only has efficient adsorption capacity for ordinary metal ions, but also exhibits efficient processing capacity for Cr2O72-. And MT3 has a better adsorption effect on ordinary metal ions. (2). Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NotI site found at 1717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1566
    Illegal BamHI site found at 2211
    Illegal BamHI site found at 2466
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1308
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961