Difference between revisions of "Part:BBa K5136226"

Revision as of 16:52, 30 September 2024

I0500-B0034-inpnc-linker-mt3-linker-mt2a-B0015

Biology

INPNC

Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).

MT3 and MT2A

The metallothioneins (MTs) are a class of low molecular weight and cysteine-rich metal binding proteins, and each one of them can bind to 6-9 heavy metal ions. The MTs are expressed as intracellular protein and are primarily responsible for metal regulation in cells of living organisms. General MTs can widely non-covalently bind divalent heavy metal ions, such as Zn²⁺, N²⁺, Pb²⁺, Hg²⁺, Cd²⁺, as well as As³⁺, but their effectiveness in treating Cr₂O₇^2- is not satisfactory. MT2a and MT3 are metallothioneins(MTs) found in Homo sapiens. MT2A not only has efficient adsorption capacity for ordinary metal ions, but also exhibits efficient processing capacity for Cr₂O₇^2-. And MT3 has a better adsorption effect on ordinary metal ions. (2). Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
Illegal NotI site found at 1717
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 1566
Illegal BamHI site found at 2211
Illegal BamHI site found at 2466
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1308
Illegal NgoMIV site found at 1641
Illegal AgeI site found at 979
Illegal AgeI site found at 1665
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 8: / Line 8: @@
 ====MT3 and MT2A====
-The metallothioneins (MTs) are a class of low molecular weight and cysteine-rich metal binding proteins, and each one of them can  bind to 6-9 heavy metal ions. The MTs are expressed as intracellular protein and are primarily responsible for metal regulation in cells of living organisms. General MTs can widely non-covalently bind divalent heavy metal ions, such as Zn<sup>2+</sup>, N<sup>2+</sup>, Pb<sup>2+</sup>, Hg<sup>2+</sup>, Cd<sup>2+</sup>, as well as As<sup>3+</sup>, but their effectiveness in treating Cr2O72- is not satisfactory. MT2a and MT3 are metallothioneins(MTs) found in Homo sapiens. MT2A not only has efficient adsorption capacity for ordinary metal ions, but also exhibits efficient processing capacity for Cr<sub>2</sub>O<sub>7</sub><sup>2-</sup>. And  MT3  has a better adsorption effect on ordinary metal ions. (2).
+The metallothioneins (MTs) are a class of low molecular weight and cysteine-rich metal binding proteins, and each one of them can  bind to 6-9 heavy metal ions. The MTs are expressed as intracellular protein and are primarily responsible for metal regulation in cells of living organisms. General MTs can widely non-covalently bind divalent heavy metal ions, such as Zn<sup>2+</sup>, N<sup>2+</sup>, Pb<sup>2+</sup>, Hg<sup>2+</sup>, Cd<sup>2+</sup>, as well as As<sup>3+</sup>, but their effectiveness in treating Cr<sub>2</sub>O<sub>7</sub><sup>2-</sup> is not satisfactory. MT2a and MT3 are metallothioneins(MTs) found in Homo sapiens. MT2A not only has efficient adsorption capacity for ordinary metal ions, but also exhibits efficient processing capacity for Cr<sub>2</sub>O<sub>7</sub><sup>2-</sup>. And  MT3  has a better adsorption effect on ordinary metal ions. (2).
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 ===Usage and Biology===