Difference between revisions of "Part:BBa K243028:Design"
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===Design Notes=== | ===Design Notes=== | ||
The cloning steps were planned theoretically before we started the work in the wet lab. The combination of split linker, Fos and Fok_a allows an alternative method to bind our universal endonuclease protein to the DNA. | The cloning steps were planned theoretically before we started the work in the wet lab. The combination of split linker, Fos and Fok_a allows an alternative method to bind our universal endonuclease protein to the DNA. | ||
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| + | [https://static.igem.org/mediawiki/parts/8/8a/Freiburg09_Fos-Split-Fok_as.txt Commented GenBank file] | ||
===Source=== | ===Source=== | ||
Latest revision as of 00:14, 22 October 2009
FOS-Split Linker-Fok_a
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 19
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 766
Design Notes
The cloning steps were planned theoretically before we started the work in the wet lab. The combination of split linker, Fos and Fok_a allows an alternative method to bind our universal endonuclease protein to the DNA.
Source
Combined by serial cloning steps.