Difference between revisions of "Part:BBa K5317017"

(→‎Theoretical Part Design)
(→‎Characterization)
Line 15: Line 15:
  
 
=Characterization=  
 
=Characterization=  
−
For further analysing co-expression experiments with PknB (<span class="plainlinks">[https://parts.igem.org/Part:BBa_K5317013 K5317013]</span>) and the ATF2-3xCre3xAP1 promoter (<span class="plainlinks">[https://parts.igem.org/Part:BBa_K5317017 K5317017]</span>) please visit the registry entry (<span class="plainlinks">[https://parts.igem.org/Part:BBa_K5317022 K5317022]</span>).  
+
For further analysing co-expression experiments with PknB (<span class="plainlinks">[https://parts.igem.org/Part:BBa_K5317013 K5317013]</span>) and the ATF2 (<span class="plainlinks">[https://parts.igem.org/Part:BBa_K5317017 K5317017]</span>) please visit the registry entry (<span class="plainlinks">[https://parts.igem.org/Part:BBa_K5317016 K5317016]</span>).
−
 
+
  
 
=References=
 
=References=

Revision as of 15:53, 30 September 2024

3xCre3xAP1-miniCMV Promoter

Usage and Biology

ATF2 belongs to the ATF/CREB family and regulates genes involved in cell growth, stress responses and apoptosis. With the addition of the ATF2 gene, this plasmid enables the study of transcriptional regulation of ATF2 (Kirsch et al., 2020) and its phosphorylation by PknB, making it important for research into signaling pathways related to cell stress and survival (Zhang et al., 2020). The consensus sequence for the CRE (cAMP-responsive elements) sequence that ATF2 binds to is typically represented as 5'-GTGACGT[AC][AG]-3' (Hai et al.,1989).

Cloning

Theoretical Part Design

This synthetic part was synthesised by Eurofins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 273

Characterization

For further analysing co-expression experiments with PknB (K5317013) and the ATF2 (K5317017) please visit the registry entry (K5317016).

References

Hai, T. W., Liu, F., Coukos, W. J., & Green, M. R. (1989). Transcription factor ATF cDNA clones: An extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers. Genes & Development, 3(12b), 2083–2090. https://doi.org/10.1101/gad.3.12b.2083

Miller, M., Donat, S., Rakette, S., Stehle, T., Kouwen, T. R. H. M., Diks, S. H., Dreisbach, A., Reilman, E., Gronau, K., Becher, D., Peppelenbosch, M. P., Van Dijl, J. M., & Ohlsen, K. (2010). Staphylococcal PknB as the First Prokaryotic Representative of the Proline-Directed Kinases. PLoS ONE, 5(2), e9057. https://doi.org/10.1371/journal.pone.0009057