Difference between revisions of "Part:BBa K5036011"
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− | after the fusion of the two parts of dCas9, it will guide the (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog | + | after the fusion of the two parts of dCas9, it will guide the (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene which is located upstream to of YAP-1 so that they can initiate transcription and boost YAP-1 production. |
+ | <html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style=" max-width:850px; | ||
+ | width:75%; | ||
+ | height:auto; | ||
+ | position: relative; | ||
+ | top: 50%; | ||
+ | left: 35%; | ||
+ | transform: translate( -50%); | ||
+ | padding-bottom:25px; | ||
+ | padding-top:25px; | ||
+ | "src="https://static.igem.wiki/teams/5036/assets/tf-1.png | ||
+ | "> | ||
+ | <p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span | ||
+ | lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of Nanog gRNA where it direct (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene for boosing YAP-1 production | ||
+ | . </span></p></div></html> | ||
Revision as of 15:49, 30 September 2024
Nanog gRNA2 of dCas9
Part Description
It is guide RNA which is a crucial component of the CRISPR-Cas9 gene editing system as it act as A guide the dCas9 to its target. It consist of two main parts: first Spacer Sequence: which is a short, user-defined sequence that complements the target DNA sequence you want to edit. Second Scaffold Sequence which is pre-designed RNA structure allows gRNA to bind to the Cas9 protein and facilitate its interaction with the target DNA
Usage
after the fusion of the two parts of dCas9, it will guide the (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene which is located upstream to of YAP-1 so that they can initiate transcription and boost YAP-1 production.
this figure illustrates the structure of Nanog gRNA where it direct (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene for boosing YAP-1 production .
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]