Difference between revisions of "Part:BBa K5036010"
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==Usage==
 
==Usage==
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after the fusion of the two parts of dCas9, it will guide the (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog enhancer of YAP-1 for interaction and initiation of transcription
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after the fusion of the two parts of dCas9, it will guide the (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene which is located upstream to of YAP-1 so that they can initiate transcription and boost YAP-1 production.
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<html><div align="center"style="border:solid #17252A; width:100%;float:center;"><img style="                              max-width:850px;
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width:75%;
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height:auto;
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position: relative;
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top: 50%;
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left: 35%;
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transform: translate( -50%);
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padding-bottom:25px;
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padding-top:25px;
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"src="https://static.igem.wiki/teams/5036/assets/tf-1.png
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">
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<p class=MsoNormal align=center style='text-align:left;border:none;width:98% ;justify-content:center;'><span
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lang=EN style='font-size:11.0pt;line-height:115%'>this figure illustrates the structure of Nanog gRNA where it direct (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene for boosing YAP-1 production
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.  </span></p></div></html>
  
  

Revision as of 15:48, 30 September 2024


Nanog gRNA1 of dCas9

Part Description

It is guide RNA which is a crucial component of the CRISPR-Cas9 gene editing system as it acts as A guide the dCas9 to its target. It consists of two main parts: first Spacer Sequence: which is a short, user-defined sequence that complements the target DNA sequence you want to edit. Second Scaffold Sequence which is pre-designed RNA structure allows gRNA to bind to the Cas9 protein and facilitate its interaction with the target DNA

Usage

after the fusion of the two parts of dCas9, it will guide the (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene which is located upstream to of YAP-1 so that they can initiate transcription and boost YAP-1 production.

this figure illustrates the structure of Nanog gRNA where it direct (dCas9, VP64, GAL4, UAS Trans CMV enhancer) complex to the target DNA of Nanog gene for boosing YAP-1 production .


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]