Difference between revisions of "Part:BBa K5299205:Design"

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===Design Notes===
 
===Design Notes===
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Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.
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Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.
  
 
===Source===
 
===Source===
  
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The promoter BBa_J45992 alrady existed in the Registry. The RBS BBa_B0034 already existed in the Registry. The sfGFP BBa_I746916 alrady existed in the Registry. The terminator BBa_B0015 already existed in the Registry.  
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The promoter BBa_J45992 already existed in the Registry. The RBS BBa_B0034 already existed in the Registry. The sfGFP BBa_I746916 alrady existed in the Registry. The terminator BBa_B0015 already existed in the Registry.
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===References===
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Latest revision as of 15:44, 30 September 2024


BBa_J45992 - BBa_B0034 - BBa_I746916 - BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 225


Design Notes

Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.

Source

The promoter BBa_J45992 already existed in the Registry. The RBS BBa_B0034 already existed in the Registry. The sfGFP BBa_I746916 alrady existed in the Registry. The terminator BBa_B0015 already existed in the Registry.