Difference between revisions of "Part:BBa K5322011:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence. | |
===Source=== | ===Source=== | ||
− | + | The team obtains the SOD-1 sequence from BBa_K2215003. | |
− | + | ||
===References=== | ===References=== |
Revision as of 15:43, 30 September 2024
pET-29(a)-pT7-lac operator-(SOD-1+His)-T7
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 577
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To ensure the successful expression of SOD-1 in Escherichia coli, we performed codon optimization of the SOD-1 sequence.
Source
The team obtains the SOD-1 sequence from BBa_K2215003.