Difference between revisions of "Part:BBa K5299203:Design"
Mkakadiari (Talk | contribs) (→Design Notes) |
Mkakadiari (Talk | contribs) (→References) |
||
| (2 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | Constructed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level | + | Constructed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs. |
===Source=== | ===Source=== | ||
The promoter BBa_J23119 already existed in the Registry. The RBS BBa_B0034 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The terminator BBa_B0015 already existed in the Registry. | The promoter BBa_J23119 already existed in the Registry. The RBS BBa_B0034 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The terminator BBa_B0015 already existed in the Registry. | ||
| − | |||
| − | |||
Latest revision as of 15:36, 30 September 2024
BBa_J23119 - BBa_B0034 - BBa_I746916 - BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 61
Design Notes
Constructed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.
Source
The promoter BBa_J23119 already existed in the Registry. The RBS BBa_B0034 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The terminator BBa_B0015 already existed in the Registry.