Difference between revisions of "Part:BBa K5299201:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Constructed according to Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level | + | Constructed according to Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs. |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
*[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. <i> Plasmid, 109 </i>(102491), 102491. doi:10.1016/j.plasmid.2020.102491 | *[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. <i> Plasmid, 109 </i>(102491), 102491. doi:10.1016/j.plasmid.2020.102491 | ||
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Latest revision as of 15:25, 30 September 2024
P3.1 - BBa_B0034 - BBa_I746916 - BBa_B0015
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 256
Design Notes
Constructed according to Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.
Source
The promoter, BBa_K4583008 is already in the Registry. It originally comes from Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491. The RBS, BBa_B0034 already exists in the Registry. The sfGFP, BBa_I746916, already exists in the Registry. The terminator, BBa_B0015, already exists in the Registry.
References
- [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491