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===Usage and Biology===
 
===Usage and Biology===
  
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We select PcW as the replacing promoter of <i>hok</i>/<i>mok</i> through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of <i>sok</i> is stronger than <i> hok</i>/<i>mok</i> when there is citrate in the environment, preventing cell death in advanced.
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Revision as of 14:20, 30 September 2024


PcW

A common weak constitutive promoter in Pseudomonas aeruginosa.

Usage and Biology

We select PcW as the replacing promoter of hok/mok through paper research for its weak function of starting genetic expression. By this way we are able to secure the expression intensity of sok is stronger than hok/mok when there is citrate in the environment, preventing cell death in advanced.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]