Difference between revisions of "Part:BBa K3064030"

Revision as of 14:07, 30 September 2024

miniPromoter miniPromoter Shortened version of minimum TATA-box Promoter. The miniPromoter shows low levels of transcription at baseline.

Usage and Biology

Sequence and Features

Improvement from the iGEM-team NUDT 2024

Usage and Biology

A promoter is a DNA sequence located in the upstream region of the 5' terminal of a structural gene, which activates RNA polymerase, makes it bind to template DNA accurately and has transcriptional initiation specificity. The general structure of promoters includes core promoter elements and upstream regulatory elements. The core promoter is usually composed of short conserved sequences such as transcription start sites and TATA Box, CAAT Box, BRE element, DPE element and MTE element, etc. [1] The structural characteristics of these sequences determine the recognition, binding and initiation of transcription of promoter and RNA polymerase. Upstream regulatory elements can change Bthe efficiency of transcription by combining with corresponding action factors.

The minimal promoter (Pmin) is usually composed of the basic TATA box, GC box and CAAT box of the promoter. Transfected vectors containing only Pmin will have a lower level of background expression when transfected into cells. But their promoter region does not contain other transcription factor binding sites, the constructed signaling pathway containing Pmin can specifically characterize whether a certain signaling pathway is activated or not.

Motivation

In order to verify the influence of CRE（cAMP response element） sequence on downstream pathway, we constructed Pmin using TATA box and GC box of CMW promoter. The Pmin is a low expression synthetic promoter in mammalian cells. CRE sequences can be placed upstream of this promoter to produce high expression of downstream genes

Reference

[1] A. Barroso-delJesus et al., "Embryonic stem cell-specific miR302-367 cluster: human gene structure and functional characterization of its core promoter," Mol Cell Biol, vol. 28, no. 21, pp. 6609-19, Nov 2008, doi: 10.1128/MCB.00398-08.