Difference between revisions of "Part:BBa K079031:Design"

 
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===References===
 
===References===
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1) JISHAGE M et al. A stationary phase protein in Escherichia coli with binding activity to the major s subunit of RNA polymerase (transcriptase controlystationary phase controlyenzyme activity control RNA polymerase storage). Proc. Natl. Acad. Sci. USA. 1998.
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2) Ishihama A. Adaptation of gene expression in stationary phase bacteria. Current Opinion in Genetics & Development 1997.

Revision as of 23:57, 21 October 2009

GFP reporter protein under the control of the BBa_J23118 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


Design Notes

The Bologna team for iGEM2008 developed the Visual Fluo software for fluorescence analysis. To test the sensitivity of the sotware we cloned the GFP reporter under the control of three promoters from the Berkley library with three different transcriptional strengths: BBa_J23105, BBa_J23118 and BBa_J23110.


Source

Registry standard parts

References

1) JISHAGE M et al. A stationary phase protein in Escherichia coli with binding activity to the major s subunit of RNA polymerase (transcriptase controlystationary phase controlyenzyme activity control RNA polymerase storage). Proc. Natl. Acad. Sci. USA. 1998. 2) Ishihama A. Adaptation of gene expression in stationary phase bacteria. Current Opinion in Genetics & Development 1997.