Difference between revisions of "Part:BBa K5301001"
Guanjiaxin (Talk | contribs) |
Guanjiaxin (Talk | contribs) (→Usage and Biology) |
||
| Line 7: | Line 7: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
| − | We | + | We devised the fusion expression of SdyCatcher with spNW15, SpyCatcher and mCherry[1-10] (namely SCSdC-mCh[1-10]). We employed SdyTag and SdyCatcher to link two proteins - SCSdC-mCh[1-10] and SnCSdT. |
===Characterization=== | ===Characterization=== | ||
Revision as of 13:12, 30 September 2024
SdyCatcher can achieve covalent binding of proteins through Tag-Catcher interaction.
SdyCatcher is a fibronectin-binding protein. It can cooperate with SdyTag to achieve covalent binding of proteins through Tag-Catcher interaction. SdyCatcher and SdyTag can also be used alongside SpyTag-SpyCatcher partners, to demonstrate kinetically controlled directed protein ligation, domain specific protein circularization and macromolecular assembly [1].
Usage and Biology
We devised the fusion expression of SdyCatcher with spNW15, SpyCatcher and mCherry[1-10] (namely SCSdC-mCh[1-10]). We employed SdyTag and SdyCatcher to link two proteins - SCSdC-mCh[1-10] and SnCSdT.
Characterization
We used SDS-PAGE to test whether this part worked.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10
Illegal AgeI site found at 58 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 55
Illegal BsaI.rc site found at 256
- ↑ Tan, L.L., S.S. Hoon and F.T. Wong, Kinetic Controlled Tag-Catcher Interactions for Directed Covalent Protein Assembly. PLOS ONE, 2016. 11(10): p. e0165074.