Difference between revisions of "Part:BBa K5293016"
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pHREAC plasmid backbone for chloroplaste targeted protein expression. Sap I sites are used to clone you insert (GGC & TGA are the scars).
 
pHREAC plasmid backbone for chloroplaste targeted protein expression. Sap I sites are used to clone you insert (GGC & TGA are the scars).
−
This empty plasmid bacbone contains an insert not displayed on the sequence for easier cloning with mCherry as a screening tool
+
This empty plasmid backbone contains an insert not displayed on the sequence for easier cloning with mCherry (BBa_K5293020) as a screening tool
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:34, 30 September 2024


pHREAC_eGFP_CHL

pHREAC plasmid backbone for chloroplaste targeted protein expression. Sap I sites are used to clone you insert (GGC & TGA are the scars). This empty plasmid backbone contains an insert not displayed on the sequence for easier cloning with mCherry (BBa_K5293020) as a screening tool

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal XbaI site found at 922
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
    Illegal PstI site found at 10399
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal NheI site found at 3235
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
    Illegal PstI site found at 10399
    Illegal NotI site found at 5724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal BglII site found at 1289
    Illegal BglII site found at 2552
    Illegal BglII site found at 2572
    Illegal BglII site found at 9342
    Illegal BamHI site found at 928
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal XbaI site found at 922
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
    Illegal PstI site found at 10399
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal XbaI site found at 922
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
    Illegal PstI site found at 10399
    Illegal NgoMIV site found at 3036
    Illegal NgoMIV site found at 4194
    Illegal NgoMIV site found at 4720
    Illegal NgoMIV site found at 5596
    Illegal NgoMIV site found at 5720
    Illegal NgoMIV site found at 6747
    Illegal NgoMIV site found at 7338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.