Difference between revisions of "Part:BBa K5097013"

 
 
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<partinfo>BBa_K5097013 short</partinfo>
 
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===Usage and Biology===
  
 
This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon.  The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification.  This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.
 
This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon.  The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification.  This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.
  
 
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===Usage and Biology===
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 11:57, 30 September 2024


BBa_K5097013 6X His tag with AGVG linker

Usage and Biology

This basic part codes for an 6X Histidine tag with a N-terminal AGVG linker and C terminal double stop codon. The part is intended for use in protein purification using IMAC (Immobilized Metal ion Affinity Chromatography) purification. This part can be added to the C-terminal of a coding sequence (with the native stop codon removed), which will add the flexible AGVG linker and then a 6X Histidine tag.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]