Difference between revisions of "Part:BBa K5097016"

 
 
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The 2024 Oneonta iGEM team pHish and CHIPs used sBFP2 as a reporter protein to test the function of pH sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of sBFP2. To do this, we generated a constitutively expressed sBFP2 (BBa_K156010) that is modified on the C-terminal to remove the native stop codon and added a AGVG linker and 6x His tag.  A double-stop codon was added at the end.
 
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===Usage and Biology===
 
===Usage and Biology===
  
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The 2024 Oneonta iGEM team pHish and CHIPs used sBFP2 as a reporter protein to test the function of pH-sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of sBFP2. To do this, we generated a constitutively expressed sBFP2 (BBa_K156010) modified on the C-terminal to remove the native stop codon and added an AGVG linker and 6x His tag.  A double-stop codon was added at the end.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 11:55, 30 September 2024


sBFP with 6X His

Usage and Biology

The 2024 Oneonta iGEM team pHish and CHIPs used sBFP2 as a reporter protein to test the function of pH-sensitive regulatory elements that control gene expression. As a companion to these studies, we investigated what effect pH might have on the fluorescence of sBFP2. To do this, we generated a constitutively expressed sBFP2 (BBa_K156010) modified on the C-terminal to remove the native stop codon and added an AGVG linker and 6x His tag. A double-stop codon was added at the end.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 91