Difference between revisions of "Part:BBa K187090"

Revision as of 23:39, 21 October 2009

terminator forward strand B, Biobytes assembly method

This part can be annealed to the terminator reverse strand (BBa_K187077), to produce a B overhang, which can anneal to the B' end on the 3' end of a part produced from pAB.

The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct.

For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or <a href="http://bbf.openwetware.org/RFC.html#BBF_RFC_47:_BioBytes_Assembly_Standard"> RFC 47 </a>

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K187090 short</partinfo>
@@ Line 7: / Line 6: @@
 The biobytes assembly method can be used to rapidly assemble DNA segments in a particular order. The 3' end of constructs made with this method can be annealed to a terminator segment, which can be digested and annealed to the anchor to circularize the construct.
-For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or RFC 47
+For more details see http://2009.igem.org/Team:Alberta/DNAanchor, or <a href="http://bbf.openwetware.org/RFC.html#BBF_RFC_47:_BioBytes_Assembly_Standard"> RFC 47 </a>
 <!-- Add more about the biology of this part here