Difference between revisions of "Part:BBa K5415004:Design"

(→‎Design Notes)
 
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<partinfo>BBa_K5415004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5415004 SequenceAndFeatures</partinfo>
  
 +
<h5>ADH1 terminator:</h5>
 +
<p>ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgt</p>
 +
<p>tttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgc</p>
  
−
===Source===
+
<h5>HIF-1beta:</h5>
 +
<p>ctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattcttcattgttgtagctgttgctctgatctcccagcatgga</p>
 +
<p>cagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatgaggctgctggcc</p>
 +
<p>ctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggt</p>
 +
<p>gagactagggtaggcagcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaa</p>
 +
<p>ctgggaagtacgagtcttagcagtagcctgggtagccacctgctgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggt</p>
 +
<p>ggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttacaggaggggctaggccactattcctgaaatt</p>
 +
<p>ctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatccgc</p>
 +
<p>attgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggt</p>
 +
<p>tgtcacaggctgaaccacctgggaatgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgc</p>
 +
<p>cagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgtggcctctggattgtgttggagagtgtaggccgtggttcttggctaga</p>
 +
<p>gttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggttctcatccagagccattcttg</p>
 +
<p>gttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatg</p>
 +
<p>acaaaattctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgtt</p>
 +
<p>gtgtcgggagatgaactctgttggttgacaaacattactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaa</p>
 +
<p>cttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccaggccctgatgtagcctgtgcagtggaccaccacgaagtg</p>
 +
<p>aggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagagctactgccaca</p>
 +
<p>cctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggat</p>
 +
<p>acgccctgtcagggcattttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattc</p>
 +
<p>agactgtggctggttcaaaacaggagtcacggagtcagacacatacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctc</p>
 +
<p>caagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttgccagttccccgcaaggacttcatgtgaga</p>
 +
<p>aactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatctt</p>
 +
<p>gttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgtt</p>
 +
<p>cccttctccatcatcatcaaaatccagccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccaga</p>
 +
<p>ggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggcagtagtcgccgccat</p>
  
−
We utilised several key iGEM BioBricks including: HIF-1 beta and HIF-1 alpha
+
<h5>GAL1,10 promoter:</h5>
 +
<p>cgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacac</p>
 +
<p>aacatatctaatcttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaa</p>
 +
<p>ctgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtc</p>
 +
<p>gcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcag</p>
 +
<p>taacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatc</p>
 +
<p>agcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattactt</p>
 +
<p>cttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaa</p>
  
−
===References===
+
<h5>HIF-1alpha</h5>
 +
<p>atggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaaagaaaagtctcgtgatgcagccagatctcggcgaagtaaagaa</p>
 +
<p>tctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgaggcttaccatcagctat</p>
 +
<p>ttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggtttt</p>
 +
<p>gttatggttctcacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtg</p>
 +
<p>tttgattttactcatccatgtgaccatgaggaaatgagagaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcga</p>
 +
<p>agtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgcaacatggaaggtattgcactgcacaggccacatt</p>
 +
<p>cacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctcacccatca</p>
 +
<p>aatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatggga</p>
 +
<p>tatgagccagaagaacttttaggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaa</p>
 +
<p>ggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtggatatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactct</p>
 +
<p>caaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttcaacaaacagaatgtgtccttaaaccg</p>
 +
<p>gttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgct</p>
 +
<p>ttaactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtacca</p>
 +
<p>ttatataatgatgtaatgctcccctcacccaacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccactt</p>
 +
<p>cgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaatccagagtcactggaactttcttttaccatgccccagattcag</p>
 +
<p>gatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgtggatagtgatatggtc</p>
 +
<p>aatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatg</p>
 +
<p>ttagctccctatatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagt</p>
 +
<p>cctcaaagcacagttacagtattccagcagactcaaatacaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtg</p>
 +
<p>acaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataaagaaactacaagtgccacatcatcaccatataga</p>
 +
<p>gatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgttatctgtc</p>
 +
<p>gctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggt</p>
 +
<p>tcactttttcaagcagtaggaattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaa</p>
 +
<p>tctagtgaacagaatggaatggagcaaaagacaattattttaataccctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggatta</p>
 +
<p>ccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaagaattactcagagctttggatcaagtt</p>
 +
<p>aacCACCACCACCATCACCACtga</p>
 +
 
 +
<h5>ADH2 terminator</h5>
 +
<p>gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaa</p>
 +
<p>taaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtaga</p>
 +
<p>catggtagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat</p>

Latest revision as of 11:36, 30 September 2024


HIF1 system for yeast humanisation.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3244
    Illegal BglII site found at 3452
    Illegal BamHI site found at 3355
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 929
    Illegal NgoMIV site found at 3383
    Illegal AgeI site found at 2972
    Illegal AgeI site found at 4459
  • 1000
    COMPATIBLE WITH RFC[1000]
ADH1 terminator:

ccggtagaggtgtggtcaataagagcgacctcatactatacctgagaaagcaacctgacctacaggaaagagttactcaagaataagaattttcgt

tttaaaacctaagagtcactttaaaatttgtatacacttattttttttataacttatttaataataaaaatcataaatcataagaaattcgc

HIF-1beta:

ctaGTGGTGATGGTGGTGGTGttctgaaaaggggggaaacatagttagatcaggaaattcttcattgttgtagctgttgctctgatctcccagcatgga

cagcatctcctggaagacctcaggctggccaggttgctgtgctggcggttgttgaacatgttgctcactagaacttgaacgatgatgaggctgctggcc

ctgccactgtggccagacacccacaccctctgctgtccgtgtctggaattgtccggcagtctgtccagtctcaggagcaaagttagatccacgattggt

gagactagggtaggcagcagcaccaggcgatgcagttggggcaccagggagggacatggagctgaaggaggatggagtctgaaagctgcccacaccaaa

ctgggaagtacgagtcttagcagtagcctgggtagccacctgctgggcagaaaagcctgagcgggtagtaggggtccaagttggggttgctccttgggt

ggggttggagtggcgggaaatctgggccaacatctgtcccgcagaagctgatggctggacaatggttacaggaggggctaggccactattcctgaaatt

ctctgccggccggggggtaggagggaatgtgttgccctgggagaatagctgttgggtggcagggacagtgctggaggagatgcctttactctgatccgc

attgatgttgtgatagatttctgaaaatcttggatctctatcctgggcaaataaaccatctgacttctcaaggggcttgctgtgttctggtcctgtggt

tgtcacaggctgaaccacctgggaatgattgtagctggccagtccatctcttcctggtaccatgtccaattctgtttgctgttgctgctgcctgggtgc

cagctgtcctgagcccatctccaggggtaaattagctgtgggacctagttgtggcctctggattgtgttggagagtgtaggccgtggttcttggctaga

gttcttcacattggtgttggtacagatgatgtactcaatttcatctgagtaagggttctggaaagtaaaggagctggttctcatccagagccattcttg

gttcttagaccggaaccggaacatgacagacagcacttggccttttaatttcactacctgttggaagctgtctcttagaagctgctggtcttcaggatg

acaaaattctacaatattctttcctaagagttcctgtggctggtagccaacagtagccacacagcggtgatccacaaaagtgaagataccctcaatgtt

gtgtcgggagatgaactctgttggttgacaaacattactcatgtctgtacagttgggagaactggttacctgcaatctgccaatggccactaggcaaaa

cttgcttccctggccagcctctgggtcatcatctgggagggaaacacctgctgggggccaggccctgatgtagcctgtgcagtggaccaccacgaagtg

aggttccccatcctttacagagccaagtccattcctgcatctgttcctcacaaagctcagcctattcacagaaactgggtccacagagctactgccaca

cctcattcggcaaataaacgatctccttgagcccatacacattctcatggaagactgctgaccttcctttttcactgttccagtctttagatccaggat

acgccctgtcagggcattttctgaagtggaaagctgctcacgaagtttatccacatcatctgggtgcacctgatcatagagtgtgctgccaaaccattc

agactgtggctggttcaaaacaggagtcacggagtcagacacatacaccaccctgcctgtctcacatgagacaataaacagaaagccatctgctgcctc

caagatcaaatgtttcagttcctgatcagtgaggaaagacggcttataggagccatcagtggatgtgttgccagttccccgcaaggacttcatgtgaga

aactgccatgcgtaagatggttagcttgtctggttttcgagccagggcactacaggtgggtaccatatctgacagttctgtgatgtaggctgtcatctt

gttccgtcgccgccgttcaatttcactgtgattttccctggcaaaccgctccttatcgttagacatctgatcatcatcacacctcaaaaatttactgtt

cccttctccatcatcatcaaaatccagccctggtcgccgcttaatagccctctggacaatggctcctccaccttggattccaggtccagagtttccaga

ggcaatggctggacccagtgatggtacatctgatgtcatttcggggttggcagtagtcgccgccat

GAL1,10 promoter:

cgaattttcaaaaattcttactttttttttggatggacgcaaagaagtttaataatcatattacatgtgtgaaattgttatccgctcacaattccacac

aacatatctaatcttacttatatgttgtggaaatgtaaagagccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaa

ctgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtc

gcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcag

taacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatc

agcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgtccctatcagtgatagagatctccctatcagtgatagagatattactt

cttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggagaaaaaaccccggatcctattaaa

HIF-1alpha

atggagggcgccggcggcgcgaacgacaagaaaaagataagttctgaacgtcgaaaagaaaagtctcgtgatgcagccagatctcggcgaagtaaagaa

tctgaagttttttatgagcttgctcatcagttgccacttccacataatgtgagttcgcatcttgataaggcctctgtgatgaggcttaccatcagctat

ttgcgtgtgaggaaacttctggatgctggtgatttggatattgaagatgacatgaaagcacagatgaattgcttttatttgaaagccttggatggtttt

gttatggttctcacagatgatggtgacatgatttacatttctgataatgtgaacaaatacatgggattaactcagtttgaactaactggacacagtgtg

tttgattttactcatccatgtgaccatgaggaaatgagagaaatgcttacacacagaaatggccttgtgaaaaagggtaaagaacaaaacacacagcga

agtttttttctcagaatgaagtgtaccctaactagccgaggaagaactatgaacataaagtctgcaacatggaaggtattgcactgcacaggccacatt

cacgtatatgataccaacagtaaccaacctcagtgtgggtataagaaaccacctatgacctgcttggtgctgatttgtgaacccattcctcacccatca

aatattgaaattcctttagatagcaagactttcctcagtcgacacagcctggatatgaaattttcttattgtgatgaaagaattaccgaattgatggga

tatgagccagaagaacttttaggccgctcaatttatgaatattatcatgctttggactctgatcatctgaccaaaactcatcatgatatgtttactaaa

ggacaagtcaccacaggacagtacaggatgcttgccaaaagaggtggatatgtctgggttgaaactcaagcaactgtcatatataacaccaagaactct

caaccacagtgcattgtatgtgtgaattacgttgtgagtggtattattcagcacgacttgattttctcccttcaacaaacagaatgtgtccttaaaccg

gttgaatcttcagatatgaaaatgactcagctattcaccaaagttgaatcagaagatacaagtagcctctttgacaaactcaagaaggaacctgatgct

ttaactttgctggccccagccgctggagacacaatcatatctttagattttggcagcaacgacacagaaactgatgaccagcaacttgaggaagtacca

ttatataatgatgtaatgctcccctcacccaacgaaaaattacagaatataaatttggcaatgtctccattacccaccgctgaaacgccaaagccactt

cgaagtagtgctgaccctgcactcaatcaagaagttgcattaaaattagaaccaaatccagagtcactggaactttcttttaccatgccccagattcag

gatcagacacctagtccttccgatggaagcactagacaaagttcacctgagcctaatagtcccagtgaatattgtttttatgtggatagtgatatggtc

aatgagttcaagttggaattggtagaaaaactttttgctgaagacacagaagcaaagaacccattttctactcaggacacagatttagacttggagatg

ttagctccctatatcccaatggatgatgacttccagttacgttccttcgatcagttgtcaccattagaaagcagttccgcaagccctgaaagcgcaagt

cctcaaagcacagttacagtattccagcagactcaaatacaagaacctactgctaatgccaccactaccactgccaccactgatgaattaaaaacagtg

acaaaagaccgtatggaagacattaaaatattgattgcatctccatctcctacccacatacataaagaaactacaagtgccacatcatcaccatataga

gatactcaaagtcggacagcctcaccaaacagagcaggaaaaggagtcatagaacagacagaaaaatctcatccaagaagccctaacgtgttatctgtc

gctttgagtcaaagaactacagttcctgaggaagaactaaatccaaagatactagctttgcagaatgctcagagaaagcgaaaaatggaacatgatggt

tcactttttcaagcagtaggaattggaacattattacagcagccagacgatcatgcagctactacatcactttcttggaaacgtgtaaaaggatgcaaa

tctagtgaacagaatggaatggagcaaaagacaattattttaataccctctgatttagcatgtagactgctggggcaatcaatggatgaaagtggatta

ccacagctgaccagttatgattgtgaagttaatgctcctatacaaggcagcagaaacctactacagggtgaagaattactcagagctttggatcaagtt

aacCACCACCACCATCACCACtga

ADH2 terminator

gcggatctcttatgtctttacgatttatagttttcattatcaagtatgcctatattagtatatagcatctttagatgacagtgttcgaagtttcacgaa

taaaagataatattctactttttgctcccaccgcgtttgctagcacgagtgaacaccatccctcgcctgtgagttgtacccattcctctaaactgtaga

catggtagcttcagcagtgttcgttatgtacggcatcctccaacaaacagtcggttatagtttgtcctgctcctctgaatcgtatccctcgatatttctcat