Difference between revisions of "Part:BBa K5322000:Design"
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===Source=== | ===Source=== | ||
| − | + | The plasmid pET29a-J23119-RBS-Mfp3-T7 utilizes the pET29a vector, which is commonly employed for robust gene expression in Escherichia coli. The components used in this design, including the J23119 promoter and Mfp3, are derived from the standard biological parts registry of iGEM (BBa_J23119, BBa_K4854000), with the mussel foot protein Mfp3 sourced from natural marine mussels. These parts were selected for their validated functionality and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions. | |
===References=== | ===References=== | ||
Revision as of 11:03, 30 September 2024
Constitutive Mfp3 Expression System
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 210
Illegal NotI site found at 174 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To address the reduced mucus layer thickness and dysbiosis causing inflammation in patients with enteritis, we aim to resolve the dysregulation of the mucosal system from the ground up. A natural high-adhesion protein from marine mussels—mussel foot protein (Mfp)—has garnered our attention, particularly Mfp3, which is one of the proteins with the highest DOPA content among the six mussel proteins. We utilized Escherichia coli BL21(DE3) to establish a production system for Mfp, enhancing adhesion in damaged intestines and regulating gut microbiota. Consequently, we designed the plasmid pET29a-J23119-RBS-Mfp3-T7.
Source
The plasmid pET29a-J23119-RBS-Mfp3-T7 utilizes the pET29a vector, which is commonly employed for robust gene expression in Escherichia coli. The components used in this design, including the J23119 promoter and Mfp3, are derived from the standard biological parts registry of iGEM (BBa_J23119, BBa_K4854000), with the mussel foot protein Mfp3 sourced from natural marine mussels. These parts were selected for their validated functionality and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions.