Revision as of 10:08, 30 September 2024

sec-HER2nb-Fc-EGFRnb

A nanobody of HERnb-Fc-EGFRnb carrying a secretory peptide for improved secretion

Profile

• Name:sec-HER2nb-Fc-EGFRnb
• Base Pairs: 1581 bp
• Amino acid: 527 a.a
• Origin: Synthetic
• Property:

Usage and Biology

Figure The AlphaFold predicted structure of sec-HER2-Fc-EGFR. Cyan: signal peptide ssSTII1 S-13L; Red: His-tag; Green: HER2 nanobody; Grey: linker; Blue: Fc protein; Orange: EGFR nanobody.

Expression

Figure 2. Protein expression of sec-HER2-Fc-EGFR. Lane 1, Total sec-HER2-Fc-EGFR in 16℃ with 0.5mM IPTG induced fraction for 24 hours; lane 2, Total sec-HER2-Fc-EGFR in 25℃ with 0.5m IPTG induced fraction for 24 hours; lane 3, Total sec-HER2-Fc-EGFR in 30℃ with 0.5mM IPTG induced fraction for 24 hours; lane 4, Biorad Precision Plus Protein™™ Unstained Protein Standards; lane 5, Total sec-HER2-Fc-EGFR in 16℃ with uninduced fraction for 24 hours; lane 6, Total sec-HER2-Fc-EGFR in 25℃ with uninduced fraction for 24 hours; lane 7, Total sec-HER2-Fc-EGFR in 30℃ with uninduced fraction for 24 hours.​

Figure 3. Protein expression of sec-HER2-Fc-EGFR. Lane 1, Biorad Precision Plus Protein™™ Unstained Protein Standards; lane 2, Soluble sec-HER2-Fc-EGFR in 16℃ with 0.5mM IPTG induced fraction for 24 hours; lane 3, Soluble sec-HER2-Fc-EGFR in 25℃ with 0.5m IPTG induced fraction for 24 hours; lane 3, Soluble sec-HER2-Fc-EGFR 5 in 30℃ with 0.5mM IPTG induced fraction for 24 hours; lane 5, Soluble sec-HER2-Fc-EGFR 5 in 16℃ with uninduced fraction for 24 hours; lane 6, Soluble sec-HER2-Fc-EGFR in 25℃ with uninduced fraction for 24 hours; lane 7, Soluble sec-HER2-Fc-EGFR in 30℃ with uninduced fraction for 24 hours.​

Figure 4. Protein expression of sec-HER2-Fc-EGFR. Lane 1, Biorad Precision Plus Protein™™ Unstained Protein Standards; lane 2, Insoluble sec-HER2-Fc-EGFR in 16℃ with 0.5mM IPTG induced fraction for 24 hours; lane 3, Insoluble sec-HER2-Fc-EGFR in 25℃ with 0.5m IPTG induced fraction for 24 hours; lane 3, Insoluble sec-HER2-Fc-EGFR 5 in 30℃ with 0.5mM IPTG induced fraction for 24 hours; lane 5, Insoluble sec-HER2-Fc-EGFR 5 in 16℃ with uninduced fraction for 24 hours; lane 6, Insoluble sec-HER2-Fc-EGFR in 25℃ with uninduced fraction for 24 hours; lane 7, Insoluble sec-HER2-Fc-EGFR in 30℃ with uninduced fraction for 24 hours.​

Reference

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• Simmons, L. C., & Yansura, D. G. (1996). Translational level is a critical factor for the secretion of heterologous proteins in Escherichia coli. Nature biotechnology, 14(5), 629-634.

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Sequence and Features