Difference between revisions of "Part:BBa K5382110"

Revision as of 10:07, 30 September 2024

Inak-linker-z domain_protein domain that have the ability to bind to lgG

InaK is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of E. coli cells as an anchor moda, so as to enable surface display.
linker here is a short peptide used to connect the InaK and z domain.
z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG)[1]. In our project, z domain is used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1674
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 722
Illegal NgoMIV site found at 962
Illegal NgoMIV site found at 1130
Illegal AgeI site found at 517
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 692
Illegal BsaI.rc site found at 1182
Illegal SapI.rc site found at 326

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5382110 short</partinfo>
-Inak is a kind of ice nucleoprotein of Pseudomonas syringae KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of E. coli cells as an anchor moda, so as to enable surface display.<br>
+InaK is a kind of ice nucleoprotein of <i>Pseudomonas syringae</i> KCTC1832. Ice nucleoprotein is a kind of protein that can promote the formation of ice crystals in organisms. Its N-terminal domain can be tightly anchored to the surface of <i>E. coli</i> cells as an anchor moda, so as to enable surface display.<br>
-linker here is a short peptide sequence used to connect the Inak and z domains.<br>
+linker here is a short peptide used to connect the InaK and z domain.<br>
 z domain refers to a synthetic protein domain that has the ability to bind to the Fc region of immunoglobulin G (IgG)[1]. In our project, z domain is used to construct extracellular vesicles (EVs) that can display antibodies, so that these EVs can bind specific antibodies through the z domain on their surface, so as to achieve targeted delivery to specific cells or tissues